Recombinant human VEGF 165 protein

Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Product Name Alternative

Vascular endothelial growth factor A, VEGF, MVCD1, VPF

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

19.3 kDa (166aa) confirmed by MALDI-TOF

Additionnal Information

VEGF165, Vascular endothelial growth factor A, VEGF, MVCD1, VPF, ATGP1449-10 µg, ATGP1449-20 µg, ATGP1449-50 µg, ATGP1449-100 µg, ATGP1449-250 µg, ATGP1449-500 µg, ATGP1449-1 mg, ATGP1449-10, ATGP1449-20, ATGP1449-50, ATGP1449-100, ATGP1449-250, ATGP1449-500, ATGP1449-1

References & Citations

Murphy J.F., et al. (2001) FASEB J. 15:1667-1669; ; Woolard J., et al. (2004) Cancer Res. 64:7822-7835

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH8.5) containing 2mM EDTA, 0.1mM PMSF, 1mM DTT, 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_001020539

Uniprot Accession Number

P15692

Species

Human

AA Sequence

MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR