Recombinant e.coli mutM protein
MutM, also known as formamidopyrimidine DNA glycosylase, is a base excision repair enzyme which recognizes and removes a wide range of oxidized purines from correspondingly damaged DNA. This protein is nonredundant and required to rapidly remove its substrate lesions on the chromosome. In addition, it also repaired a significant portion of the lesions recognized by Endo III, suggesting that it plays a prominent role in the global repair of both purine damage and pyrimidine damage in vivo. Recombinant E. coli mutM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Formamidopyrimidine-DNA glycosylase, fpg
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
32.4 kDa (289aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Serre L. et al. (2002) EMBO J. 21:2854-2865; ; Schalow BJ. et al. (2011) J Mol Biol, 410:183-193;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl,1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_418092
Uniprot Accession Number
P05523
Species
E.coli
AA Sequence
Frequently Asked Questions
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