Recombinant e.coli mutM protein

MutM, also known as formamidopyrimidine DNA glycosylase, is a base excision repair enzyme which recognizes and removes a wide range of oxidized purines from correspondingly damaged DNA. This protein is nonredundant and required to rapidly remove its substrate lesions on the chromosome. In addition, it also repaired a significant portion of the lesions recognized by Endo III, suggesting that it plays a prominent role in the global repair of both purine damage and pyrimidine damage in vivo. Recombinant E. coli mutM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Formamidopyrimidine-DNA glycosylase, fpg

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

32.4 kDa (289aa) confirmed by MALDI-TOF

Additionnal Information

MutM, ATGP1448-10 µg, ATGP1448-20 µg, ATGP1448-50 µg, ATGP1448-100 µg, ATGP1448-250 µg, ATGP1448-500 µg, ATGP1448-1 mg, ATGP1448-10, ATGP1448-20, ATGP1448-50, ATGP1448-100, ATGP1448-250, ATGP1448-500, ATGP1448-1

References & Citations

Serre L. et al. (2002) EMBO J. 21:2854-2865; ; Schalow BJ. et al. (2011) J Mol Biol, 410:183-193;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl,1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_418092

Uniprot Accession Number

P05523

Species

E.coli

AA Sequence

MPELPEVETS RRGIEPHLVG ATILHAVVRN GRLRWPVSEE IYRLSDQPVL SVQRRAKYLL LELPEGWIII HLGMSGSLRI LPEELPPEKH DHVDLVMSNG KVLRYTDPRR FGAWLWTKEL EGHNVLTHLG PEPLSDDFNG EYLHQKCAKK KTAIKPWLMD NKLVVGVGNI YASESLFAAG IHPDRLASSL SLAECELLAR VIKAVLLRSI EQGGTTLKDF LQSDGKPGYF AQELQVYGRK GEPCRVCGTP IVATKHAQRA TFYCRQCQK