Recombinant human VPS25 protein
VPS25, also known as vacuolar protein-sorting-associated protein 25, consists of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may play a role in transcription regulation, possibly via its interaction with ELL. Recombinant human VPS25 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Vacuolar protein sorting 25 homolog, Dermal papilla-derived protein 9, DERP9, ELL-associated protein of 20 kDa, EAP20, ESCRT-II complex subunit VPS25
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
23.3 kDa (200aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Bailey, S.D. et al. (2010) Diabetes Care 33 (10), 2250-2253; ; Talmud, P.J. et al. (2009) Am. J. Hum. Genet. 85 (5), 628-642;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M Nacl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_115729
Uniprot Accession Number
Q9BRG1
Species
Human
AA Sequence
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