Recombinant human ALAD protein
The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins) . ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Recombinant human ALAD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Delta-aminolevulinic acid dehydratase, ALADH, PBGS
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
38.8 kDa (354aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Li, C.. et al. (2011) Toxicol. Lett. 203 (1), 48-53; ; van Bemmel et al. (2011) Epidemiology 22 (2), 273-278
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_000022
Uniprot Accession Number
P13716
Species
Human
AA Sequence
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