Recombinant human ALAD protein

The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins) . ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Recombinant human ALAD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Delta-aminolevulinic acid dehydratase, ALADH, PBGS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

38.8 kDa (354aa) confirmed by MALDI-TOF

Additionnal Information

ALAD, Delta-aminolevulinic acid dehydratase, ALADH, PBGS, ATGP1444-10 µg, ATGP1444-20 µg, ATGP1444-50 µg, ATGP1444-100 µg, ATGP1444-250 µg, ATGP1444-500 µg, ATGP1444-1 mg, ATGP1444-10, ATGP1444-20, ATGP1444-50, ATGP1444-100, ATGP1444-250, ATGP1444-500, ATGP1444-1

References & Citations

Li, C.. et al. (2011) Toxicol. Lett. 203 (1), 48-53; ; van Bemmel et al. (2011) Epidemiology 22 (2), 273-278

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_000022

Uniprot Accession Number

P13716

Species

Human

AA Sequence

MQPQSV LHSGYFHPLL RAWQTATTTL NASNLIYPIF VTDVPDDIQP ITSLPGVARY GVKRLEEMLR PLVEEGLRCV LIFGVPSRVP KDERGSAADS EESPAIEAIH LLRKTFPNLL VACDVCLCPY TSHGHCGLLS ENGAFRAEES RQRLAEVALA YAKAGCQVVA PSDMMDGRVE AIKEALMAHG LGNRVSVMSY SAKFASCFYG PFRDAAKSSP AFGDRRCYQL PPGARGLALR AVDRDVREGA DMLMVKPGMP YLDIVREVKD KHPDLPLAVY HVSGEFAMLW HGAQAGAFDL KAAVLEAMTA FRRAGADIII TYYTPQLLQW LKEE