Recombinant human STYX protein
STYX, also known as serine/threonine/tyrosine-interacting protein, belongs to the protein-tyrosine phosphatase family. It contains a Gly residue instead of a conserved Cys residue in the dsPTPase catalytic loop which renders it catalytically inactive as a phosphatase. The binding pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases. It seems to play a role in spermiogenesis. Recombinant human STYX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Serine/threonine/tyrosine-interacting protein, FLJ42934
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
28.0 kDa (247aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Wishart MJ. et al. (1995) J. Biol. Chem. 270:26782-26785.; ; Wishart MJ. et al. (2002) Proc Natl Acad Sci u S A. 99:2112-2117.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH8.5) containing 40% glycerol, 1mM DTT, 0.1M NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_660294
Uniprot Accession Number
Q8WUJ0
Species
Human
AA Sequence
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