Recombinant human RPL8 protein
RPL8 (60S ribosomal protein L8) belongs to the ribosomal protein L2P family. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm and exists as a component of the 60S subunit where it is thought to play a role in aminoacyl-tRNA binding, specifically at the ribosomal subunit interface. Recombinant human RPL8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Product Name Alternative
60S ribosomal protein L8, L8
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
30.1 kDa (277aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kenmochi, N., et al. (1998) Genome Res. 8: 509-523.; ; Hanes J, et al. (1994) Biochem Biophys Res Commun 197 (3) : 1223-8.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 300 mM NaCl, 2mM EDTA
Scientific Category
Signal Transduction
NCBI Accession Number
NP_000964
Uniprot Accession Number
P62917
Species
Human
AA Sequence
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