Recombinant human CMT2/MAD2L1BP protein

MAD2L1-binding protein, also known as MAD2L1BP, belongs to the MAD2L1BP family. This protein may function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. Recombinant human MAD2L1BP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

MAD2L1-binding protein, CMT2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

33.6 kDa (298aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

MAD2L1BP, MAD2L1-binding protein, CMT2, ATGP1441-10 µg, ATGP1441-20 µg, ATGP1441-50 µg, ATGP1441-100 µg, ATGP1441-250 µg, ATGP1441-500 µg, ATGP1441-1 mg, ATGP1441-10, ATGP1441-20, ATGP1441-50, ATGP1441-100, ATGP1441-250, ATGP1441-500, ATGP1441-1

References & Citations

Habu T, et al. (2002) . EMBO J. 21 (23) : 6419-28.; ; Howell BJ, et al. (2000) J Cell Biol. 18; ;150 (6) :1233-50.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100 mM NaCl

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_055443.1

Uniprot Accession Number

Q15013

Species

Human

AA Sequence

HMAAPEA EVLSSAAVPD LEWYEKSEET HASQIELLET SSTQEPLNAS EAFCPRDCMV PVVFPGPVSQ EGCCQFTCEL LKHIMYQRQQ LPLPYEQLKH FYRKPSPQAE EMLKKKPRAT TEVSSRKCQQ ALAELESVLS HLEDFFARTL VPRVLILLGG NALSPKEFYE LDLSLLAPYS VDQSLSTAAC LRRLFRAIFM ADAFSELQAP PLMGTVVMAQ GHRNCGEDWF RPKLNYRVPS RGHKLTVTLS CGRPSIRTTA WEDYIWFQAP VTFKGFRE