Recombinant human GATM protein
GATM (Glycine amidinotransferase, mitochondrial) is a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Creatine plays a vital role in energy metabolism in muscle tissues. It plays a role in embryonic and central nervous system development. Recombinant human GATM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Glycine amidinotransferase mitochondrial, glycine amidinotransferase, mitochondrial, AGAT, AT
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
46.9 kDa (410aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Schulze A (2003) . Mol. Cell. Biochem. 244 (1-2) : 143-50.; ; Cullen M.E., Et al. (2006) Circulation 114:I16-I20;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_001473.1
Uniprot Accession Number
P50440
Species
Human
AA Sequence
Frequently Asked Questions
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