Recombinant human PBK protein

Lymphokine-activated killer T-cell-originated protein kinase, also known as PBK, is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase (MAPKK) family. PBK is abundant in placenta and absent from adult brain tissue. A PDZ domain in the tumor suppressor protein Dlg can coordinate with the T/SXV motif of PBK. This protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Recombinant human PBK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Lymphokine-activated killer T-cell-originated protein kinase, CT84, Nori-3, SPK, TOPK

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

38.6 kDa (346aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

PBK, Lymphokine-activated killer T-cell-originated protein kinase, CT84, Nori-3, SPK, TOPK, ATGP1437-10 µg, ATGP1437-20 µg, ATGP1437-50 µg, ATGP1437-100 µg, ATGP1437-250 µg, ATGP1437-500 µg, ATGP1437-1 mg, ATGP1437-10, ATGP1437-20, ATGP1437-50, ATGP1437-100, ATGP1437-250, ATGP1437-500, ATGP1437-1

References & Citations

Abe Y., et al. (2000) J Biol Chem. 275:21525-21531.; ; Dougherty J D., et al. (2005) J Neurosci. 25:10773-10785.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Cell Cycle

NCBI Accession Number

NP_060962

Uniprot Accession Number

Q96KB5

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMEGISN FKTPSKLSEK KKSVLCSTPT INIPASPFMQ KLGFGTGVNV YLMKRSPRGL SHSPWAVKKI NPICNDHYRS VYQKRLMDEA KILKSLHHPN IVGYRAFTEA NDGSLCLAME YGGEKSLNDL IEERYKASQD PFPAAIILKV ALNMARGLKY LHQEKKLLHG DIKSSNVVIK GDFETIKICD VGVSLPLDEN MTVTDPEACY IGTEPWKPKE AVEENGVITD KADIFAFGLT LWEMMTLSIP HINLSNDDDD EDKTFDESDF DDEAYYAALG TRPPINMEEL DESYQKVIEL FSVCTNEDPK DRPSAAHIVE ALETDV