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Recombinant human RRAGC protein

Ras-related GTP binding C, also known as RRAGC, is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways. RRAGC has intrinsic GTPase activity. It also regulates the organization of the actin cytoskeleton. Recombinant human RRAGC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ras-related GTP binding C, GTR2, RAGC

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

46.7 kDa (423aa) confirmed by MALDI-TOF

Additionnal Information

RRAGC, Ras-related GTP binding C, GTR2, RAGC, ATGP1436-10 µg, ATGP1436-20 µg, ATGP1436-50 µg, ATGP1436-100 µg, ATGP1436-250 µg, ATGP1436-500 µg, ATGP1436-1 mg, ATGP1436-10, ATGP1436-20, ATGP1436-50, ATGP1436-100, ATGP1436-250, ATGP1436-500, ATGP1436-1

References & Citations

Sekiquchi T., et al. (2001) J Biol Chem. 276:7246-57.; ; Sekiquchi T., et al. (2004) J Biol Chem. 279:8343-50.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_071440

Uniprot Accession Number

Q9HB90

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMSLQYG AEETPLAGSY GAADSFPKDF GYGVEEEEEE AAAAGGGVGA GAGGGCGPGG ADSSKPRILL MGLRRSGKSS IQKVVFHKMS PNETLFLEST NKIYKDDISN SSFVNFQIWD FPGQMDFFDP TFDYEMIFRG TGALIYVIDA QDDYMEALTR LHITVSKAYK VNPDMNFEVF IHKVDGLSDD HKIETQRDIH QRANDDLADA GLEKLHLSFY LTSIYDHSIF EAFSKVVQKL IPQLPTLENL LNIFISNSGI EKAFLFDVVS KIYIATDSSP VDMQSYELCC DMIDVVIDVS CIYGLKEDGS GSAYDKESMA IIKLNNTTVL YLKEVTKFLA LVCILREESF ERKGLIDYNF HCFRKAIHEV FEVGVTSHRS CGHQTSASSL KALTHNGTPR NAI
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