Recombinant human RCN3 protein

Reticulocalbin 3, also known as RCN3, is a member of the CREC (cab45/reticulocalbin/ ERC45/calumenin) family. RCN3 contains five Arg-Xaa-Xaa-Arg motifs, which function as target sequences of SPCs (subtilisin-like proprotein convertases), a family of serine endoproteases that proteolytically activate proproteins. The synthesis of one such member, PACE4 (paired basic amino acid cleaving enzyme 4), is influenced by association and coexpression with reticulocalbin-3. Recombinant human RCN3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Reticulocalbin 3, RLP49

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

37.9 kDa (333aa) confirmed by MALDI-TOF

Additionnal Information

RCN3, Reticulocalbin 3, RLP49, ATGP1434-10 µg, ATGP1434-20 µg, ATGP1434-50 µg, ATGP1434-100 µg, ATGP1434-250 µg, ATGP1434-500 µg, ATGP1434-1 mg, ATGP1434-10, ATGP1434-20, ATGP1434-50, ATGP1434-100, ATGP1434-250, ATGP1434-500, ATGP1434-1

References & Citations

Teglund S., et al. (1994) Genomics. 23:669-684.; ; Tsuji A., et al. (2006) Biochem J. 396:51-59.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_065701

Uniprot Accession Number

Q96D15

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMKPSPD AGPHGQGRVH QAAPLSDAPH DDAHGNFQYD HEAFLGREVA KEFDQLTPEE SQARLGRIVD RMDRAGDGDG WVSLAELRAW IAHTQQRHIR DSVSAAWDTY DTDRDGRVGW EELRNATYGH YAPGEEFHDV EDAETYKKML ARDERRFRVA DQDGDSMATR EELTAFLHPE EFPHMRDIVI AETLEDLDRN KDGYVQVEEY IADLYSAEPG EEEPAWVQTE RQQFRDFRDL NKDGHLDGSE VGHWVLPPAQ DQPLVEANHL LHESDTDKDG RLSKAEILGN WNMFVGSQAT NYGEDLTRHH DEL