Recombinant human ADH1A protein

Alcohol dehydrogenase 1A, also known as ADH1A, belongs to the alcohol dehydrogenase family. ADH1 is a monomorphic and predominant in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood. ADH1A plays a major role in ethanol metabolism. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohols to ketones or aldehydes. The physiologic function for ADH1A in the liver is the removal of ethanol formed by microorganisms in the intestinal tract. Recombinant human ADH1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Alcohol dehydrogenase 1A, ADH1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

42 kDa (395aa) confirmed by MALDI-TOF

Additionnal Information

ADH1A, Alcohol dehydrogenase 1A, ADH1, ATGP1432-10 µg, ATGP1432-20 µg, ATGP1432-50 µg, ATGP1432-100 µg, ATGP1432-250 µg, ATGP1432-500 µg, ATGP1432-1 mg, ATGP1432-10, ATGP1432-20, ATGP1432-50, ATGP1432-100, ATGP1432-250, ATGP1432-500, ATGP1432-1

References & Citations

Jelski W., et al. (2007) Dig Dis Sci. 52:1513-1516.; ; Smith M., et al. (1973) Ann Hum Genet. 36:401-414.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_000658.1

Uniprot Accession Number

P07327

Species

Human

AA Sequence

MSTAGKVIKC KAAVLWELKK PFSIEEVEVA PPKAHEVRIK MVAVGICGTD DHVVSGTMVT PLPVILGHEA AGIVESVGEG VTTVKPGDKV IPLAIPQCGK CRICKNPESN YCLKNDVSNP QGTLQDGTSR FTCRRKPIHH FLGISTFSQY TVVDENAVAK IDAASPLEKV CLIGCGFSTG YGSAVNVAKV TPGSTCAVFG LGGVGLSAIM GCKAAGAARI IAVDINKDKF AKAKELGATE CINPQDYKKP IQEVLKEMTD GGVDFSFEVI GRLDTMMASL LCCHEACGTS VIVGVPPDSQ NLSMNPMLLL TGRTWKGAIL GGFKSKECVP KLVADFMAKK FSLDALITHV LPFEKINEGF DLLHSGKSIR TILMF