Recombinant human ATG4B protein

ATG4B, also known as cysteine protease ATG4B, is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II) . Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE) -conjugated form, and has the capacity for the binding to autophagosomes. Recombinant human ATG4B protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Cysteine protease ATG4B, AuTL1, Autophagin-1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

45.3 kDa (401aa) confirmed by MALDI-TOF

Additionnal Information

ATG4B, Cysteine protease ATG4B, AuTL1, Autophagin-1, ATGP1431-10 µg, ATGP1431-20 µg, ATGP1431-50 µg, ATGP1431-100 µg, ATGP1431-250 µg, ATGP1431-500 µg, ATGP1431-1 mg, ATGP1431-10, ATGP1431-20, ATGP1431-50, ATGP1431-100, ATGP1431-250, ATGP1431-500, ATGP1431-1