Recombinant human Glutathione Peroxidase 3/GPX3 (U73C) protein

GPX3, also known as glutathione peroxidase 3, belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. GPX3 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. Recombinant human GPX3 (u73C) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography

Product Specifications

Product Name Alternative

Glutathione peroxidase 3, GPx-P, GSHPx-3, GSHPx-P, Extracellular glutathione peroxidase, Plasma glutathione peroxidase, GPXP, Selenoprotein GPX3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.7 kDa (227aa) confirmed by MALDI-TOF

Additionnal Information

Glutathione Peroxidase3, GPX3 (u73C), Glutathione peroxidase 3, GPx-P, GSHPx-3, GSHPx-P, ATGP1429-10 µg, ATGP1429-20 µg, ATGP1429-50 µg, ATGP1429-100 µg, ATGP1429-250 µg, ATGP1429-500 µg, ATGP1429-1 mg, ATGP1429-10, ATGP1429-20, ATGP1429-50, ATGP1429-100, ATGP1429-250, ATGP1429-500, ATGP1429-1

References & Citations

Esworthy R.S., et al. (1991) Arch. Biochem. Biophys. 286:330-336; ; Comhair S.A.A., et al. (2000) Am. J. Respir. Cell Mol. Biol. 23:350-354

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT, 50 mM Imidazole

Scientific Category

Redox Proteins

NCBI Accession Number

NP_002075

Uniprot Accession Number

P22352

Species

Human

AA Sequence

QSRGQEKSK MDCHGGISGT IYEYGALTID GEEYIPFKQY AGKYVLFVNV ASYCGLTGQY IELNALQEEL APFGLVILGF PCNQFGKQEP GENSEILPTL KYVRPGGGFV PNFQLFEKGD VNGEKEQKFY TFLKNSCPPT SELLGTSDRL FWEPMKVHDI RWNFEKFLVG PDGIPIMRWH HRTTVSNVKM DILSYMRRQA ALGVKRK