Recombinant human Glutathione Peroxidase 3/GPX3 (U73C) protein
GPX3, also known as glutathione peroxidase 3, belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. GPX3 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. Recombinant human GPX3 (u73C) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography
Product Specifications
Product Name Alternative
Glutathione peroxidase 3, GPx-P, GSHPx-3, GSHPx-P, Extracellular glutathione peroxidase, Plasma glutathione peroxidase, GPXP, Selenoprotein GPX3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.7 kDa (227aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Esworthy R.S., et al. (1991) Arch. Biochem. Biophys. 286:330-336; ; Comhair S.A.A., et al. (2000) Am. J. Respir. Cell Mol. Biol. 23:350-354
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT, 50 mM Imidazole
Scientific Category
Redox Proteins
NCBI Accession Number
NP_002075
Uniprot Accession Number
P22352
Species
Human
AA Sequence
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