Recombinant human IkB-beta protein

NFKBIB, also known as NF-kappa-B inhibitor beta, belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Recombinant human NFKBIB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

NF-kappa-B inhibitor beta, IKBB, TRIP9

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

40.3 kDa (380aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

IkB-b, NF-kappa-B inhibitor beta, IKBB, TRIP9, ATGP1428-10 µg, ATGP1428-20 µg, ATGP1428-50 µg, ATGP1428-100 µg, ATGP1428-250 µg, ATGP1428-500 µg, ATGP1428-1 mg, ATGP1428-10, ATGP1428-20, ATGP1428-50, ATGP1428-100, ATGP1428-250, ATGP1428-500, ATGP1428-1

References & Citations

Fenwick C., et al. (2000) Science. 287:869-873; ; DiDonato J.A., et al. (1996) Mol. Cell. Biol. 16:1295-1304

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002494

Uniprot Accession Number

Q15653

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMAGVAC LGKAADADEW CDSGLGSLGP DAAAPGGPGL GAELGPGLSW APLVFGYVTE DGDTALHLAV IHQHEPFLDF LLGFSAGTEY MDLQNDLGQT ALHLAAILGE TSTVEKLYAA GAGLCVAERR GHTALHLACR VGAHACARAL LQPRPRRPRE APDTYLAQGP DRTPDTNHTP VALYPDSDLE KEEEESEEDW KLQLEAENYE GHTPLHVAVI HKDVEMVRLL RDAGADLDKP EPTCGRSPLH LAVEAQAADV LELLLRAGAN PAARMYGGRT PLGSAMLRPN PILARLLRAH GAPEPEGEDE KSGPCSSSSD SDSGDEGDEY DDIVVHSSRS QTRLPPTPAS KPLPDDPRPV