Recombinant human UBA3 protein
UBA3, also known as NEDD8-activating enzyme E1 catalytic subunit, is the catalytic subunit of the dimeric uBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-uBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of uBE2M. Recombinant human uBA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
NEDD8-activating enzyme E1 catalytic subunit, uBE1C
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
54.4 kDa (487aa)
Additionnal Information
References & Citations
Gong L., et al. (1999) J. Biol. Chem. 274:12036-12042; ; Bohnsack R.N., et al. (2003) J. Biol. Chem. 278:26823-26830;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
Scientific Category
Ubiquitination
NCBI Accession Number
AAH22853
Uniprot Accession Number
Q8TBC4
Species
Human
AA Sequence
Frequently Asked Questions
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