Recombinant e.coli ndk protein

Ndk, also known as Nucleoside diphosphate kinase, is an enzyme that catalyzes the exchange of phosphate groups between different nucleoside diphosphates. This protein activity maintains an equilibrium between the concentrations of different nucleoside triphosphates such as, for example, when GTP produced in the citric acid (Krebs) cycle is converted to ATP. Recombinant E. coli ndk protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nucleoside diphosphate kinase, ECK2514, JW2502

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

18 kDa (167aa) confirmed by MALDI-TOF

Additionnal Information

Ndk, Nucleoside diphosphate kinase, ECK2514, JW2502, ATGP1424-10 µg, ATGP1424-20 µg, ATGP1424-50 µg, ATGP1424-100 µg, ATGP1424-250 µg, ATGP1424-500 µg, ATGP1424-1 mg, ATGP1424-10, ATGP1424-20, ATGP1424-50, ATGP1424-100, ATGP1424-250, ATGP1424-500, ATGP1424-1

References & Citations

Lee B. et al. (2009) FEBS Lett. 583:3291-3295; ; Kihara A. et al. (2011) Biosci Biotechnol Biochem, 75:1740-1745

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_417013

Uniprot Accession Number

P0A763

Species

E.coli

AA Sequence

MAIERT FSIIKPNAVA KNVIGNIFAR FEAAGFKIVG TKMLHLTVEQ ARGFYAEHDG KPFFDGLVEF MTSGPIVVSV LEGENAVQRH RDLLGATNPA NALAGTLRAD YADSLTENGT HGSDSVESAA REIAYFFGEG EVCPRTR