Recombinant human ASPA protein

ASPA, also known as aspartoacylase, is expressed in liver, lung and kidney tissue, as well as in skeletal muscle and in cerebral white matter. Existing as a homodimer, Aspartoacylase functions to catalyze the deacetylation of N-acetylaspartic acid (NAA) (a protein whose hydrolysis is crucial to maintenance of intact white matter) to produce acetate and L-aspartate. Recombinant human ASPA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Aspartoacylase, ACY2, ASP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

38.1 kDa (336aa) confirmed by MALDI-TOF

Additionnal Information

ASPA, Aspartoacylase, ACY2, ASP, ATGP1421-10 µg, ATGP1421-20 µg, ATGP1421-50 µg, ATGP1421-100 µg, ATGP1421-250 µg, ATGP1421-500 µg, ATGP1421-1 mg, ATGP1421-10, ATGP1421-20, ATGP1421-50, ATGP1421-100, ATGP1421-250, ATGP1421-500, ATGP1421-1

References & Citations

Le Coq. et al. (2006) Biochemistry 45: 5878-5884.; ; Le Coq. et al. (2008) Biochemistry 47: 3484-3492.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl, 0.1mM PMSF

Scientific Category

Neuroscience

NCBI Accession Number

NP_000040.1

Uniprot Accession Number

P45381

Species

Human

AA Sequence

MTSCHIA EEHIQKVAIF GGTHGNELTG VFLVKHWLEN GAEIQRTGLE VKPFITNPRA VKKCTRYIDC DLNRIFDLEN LGKKMSEDLP YEVRRAQEIN HLFGPKDSED SYDIIFDLHN TTSNMGCTLI LEDSRNNFLI QMFHYIKTSL APLPCYVYLI EHPSLKYATT RSIAKYPVGI EVGPQPQGVL RADILDQMRK MIKHALDFIH HFNEGKEFPP CAIEVYKIIE KVDYPRDENG EIAAIIHPNL QDQDWKPLHP GDPMFLTLDG KTIPLGGDCT VYPVFVNEAA YYEKKEAFAK TTKLTLNAKS IRCCLH