Recombinant human TARS1 protein

TARS (Threonyl-tRNA synthetase, cytoplasmic) belongs to the class-II aminoacyl-tRNA synthetase family. This protein has its main role in tRNA aminoacylation. The N-terminal domain of the enzyme is responsible for the competition with the ribosome whilst the catalytic and the C-terminal domain are involved in binding the two anticodon arm-like structures in the operator. Recombinant human TARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Threonyl-tRNA synthetase cytoplasmic, threonyl-tRNA synthetase, cytoplasmic, ThrRS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

85.6 kDa (743aa)

Additionnal Information

TDP43, TARDBP, threonyl-tRNA synthetase cytoplasmic, threonyl-tRNA synthetase, cytoplasmic, ThrRS, ATGP1417-10 µg, ATGP1417-20 µg, ATGP1417-50 µg, ATGP1417-100 µg, ATGP1417-250 µg, ATGP1417-500 µg, ATGP1417-1 mg, ATGP1417-10, ATGP1417-20, ATGP1417-50, ATGP1417-100, ATGP1417-250, ATGP1417-500, ATGP1417-1

References & Citations

Freist W, et al. (1995) . Biol. Chem. Hoppe-Seyler 376 (4) : 213-24.; ; Pan F, et al. (1982) . Int. J. Pept. Protein Res. 20 (2) : 159-66.

Other References

Wang W, et al. The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. (Nat Med. 2016) {https://pubmed.ncbi.nlm.nih.gov/27348499/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 150 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_689508

Uniprot Accession Number

P26639

Species

Human

AA Sequence

MFEEKASSPS GKMGGEEKPI GAGEEKQKEG GKKKNKEGSG DGGRAELNPW PEYIYTRLEM YNILKAEHDS ILAEKAEKDS KPIKVTLPDG KQVDAESWKT TPYQIACGIS QGLADNTVIA KVNNVVWDLD RPLEEDCTLE LLKFEDEEAQ AVYWHSSAHI MGEAMERVYG GCLCYGPPIE NGFYYDMYLE EGGVSSNDFS SLEALCKKII KEKQAFERLE VKKETLLAMF KYNKFKCRIL NEKVNTPTTT VYRCGPLIDL CRGPHVRHTG KIKALKIHKN SSTYWEGKAD METLQRIYGI SFPDPKMLKE WEKFQEEAKN RDHRKIGRDQ ELYFFHELSP GSCFFLPKGA YIYNALIEFI RSEYRKRGFQ EVVTPNIFNS RLWMTSGHWQ HYSENMFSFE VEKELFALKP MNCPGHCLMF DHRPRSWREL PLRLADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCAME QIEDEIKGCL DFLRTVYSVF GFSFKLNLST RPEKFLGDIE VWDQAEKQLE NSLNEFGEKW ELNSGDGAFY GPKIDIQIKD AIGRYHQCAT IQLDFQLPIR FNLTYVSHDG DDKKRPVIVH RAILGSVERM IAILTENYGG KWPFWLSPRQ VMVVPVGPTC DEYAQKVRQQ FHDAKFMADI DLDPGCTLNK KIRNAQLAQY NFILVVGEKE KISGTVNIRT RDNKVHGERT ISETIERLQQ LKEFRSKQAE EEF