Recombinant human TARS1 protein
TARS (Threonyl-tRNA synthetase, cytoplasmic) belongs to the class-II aminoacyl-tRNA synthetase family. This protein has its main role in tRNA aminoacylation. The N-terminal domain of the enzyme is responsible for the competition with the ribosome whilst the catalytic and the C-terminal domain are involved in binding the two anticodon arm-like structures in the operator. Recombinant human TARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Threonyl-tRNA synthetase cytoplasmic, threonyl-tRNA synthetase, cytoplasmic, ThrRS
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
85.6 kDa (743aa)
Additionnal Information
References & Citations
Freist W, et al. (1995) . Biol. Chem. Hoppe-Seyler 376 (4) : 213-24.; ; Pan F, et al. (1982) . Int. J. Pept. Protein Res. 20 (2) : 159-66.
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 150 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_689508
Uniprot Accession Number
P26639
Species
Human
AA Sequence
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