Recombinant human Livin beta protein

Livin isoform beta is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING zinc finger (RZF) domain. Livin isoform beta has direct interaction with several caspases including caspase-3, -7, and -9. This protein inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. Recombinant human Livin beta protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Livin inhibitor of apoptosis isoform beta, BIRC7, KIAP, ML-IAP, MLIAP, RNF50

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

33.4 kDa (304aa) confirmed by MALDI-TOF

Additionnal Information

Livin b, Livin inhibitor of apoptosis isoform beta, BIRC7, KIAP, ML-IAP, MLIAP, RNF50, ATGP1416-10 µg, ATGP1416-20 µg, ATGP1416-50 µg, ATGP1416-100 µg, ATGP1416-250 µg, ATGP1416-500 µg, ATGP1416-1 mg, ATGP1416-10, ATGP1416-20, ATGP1416-50, ATGP1416-100, ATGP1416-250, ATGP1416-500, ATGP1416-1

References & Citations

Kasof GM., et al. (2001) . J Biol Chem. 276 (5) :3238-46. ; ; Ashhab Y., et al. (2001) . FEBS Lett. 495 (1-2) :56-60.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 300 mM NaCl, 1mM EDTA

Scientific Category

Apoptosis

NCBI Accession Number

NP_071444

Uniprot Accession Number

Q96CA5

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMGPKDS AKCLHRGPQP SHWAAGDGPT QERCGPRSLG SPVLGLDTCR AWDHVDGQIL GQLRPLTEEE EEEGAGATLS RGPAFPGMGS EELRLASFYD WPLTAEVPPE LLAAAGFFHT GHQDKVRCFF CYGGLQSWKR GDDPWTEHAK WFPSCQFLLR SKGRDFVHSV QETHSQLLGS WDPWEEPEDA APVAPSVPAS GYPELPTPRR EVQSESAQEP GARDVEAQLR RLQEERTCKV CLDRAVSIVF VPCGHLVCAE CAPGLQLCPI CRAPVRSRVR TFLS