Recombinant human TPK1 protein

TPK1, also known as thiamin pyrophosphokinase 1, catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. This protein can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. Recombinant human TPK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Thiamin pyrophosphokinase 1, HTPK1, PP20

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

29.8 kDa (267aa) confirmed by MALDI-TOF

Additionnal Information

TPK1, Thiamin pyrophosphokinase 1, HTPK1, PP20, ATGP1415-10 µg, ATGP1415-20 µg, ATGP1415-50 µg, ATGP1415-100 µg, ATGP1415-250 µg, ATGP1415-500 µg, ATGP1415-1 mg, ATGP1415-10, ATGP1415-20, ATGP1415-50, ATGP1415-100, ATGP1415-250, ATGP1415-500, ATGP1415-1

References & Citations

Nosaka K., et al. (2001) Biochim. Biophys. Acta. 1517:293-297; ; Zhao R., et al. (2004) Biochim. Biophys. Acta. 1517:320-322

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_071890

Uniprot Accession Number

Q9H3S4

Species

Human

AA Sequence

MEHAFT PLEPLLSTGN LKYCLVILNQ PLDNYFRHLW NKALLRACAD GGANRLYDIT EGERESFLPE FINGDFDSIR PEVREYYATK GCELISTPDQ DHTDFTKCLK MLQKKIEEKD LKVDVIVTLG GLAGRFDQIM ASVNTLFQAT HITPFPIIII QEESLIYLLQ PGKHRLHVDT GMEGDWCGLI PVGQPCMQVT TTGLKWNLTN DVLAFGTLVS TSNTYDGSGV VTVETDHPLL WTMAIKS