Recombinant human TPK1 protein
TPK1, also known as thiamin pyrophosphokinase 1, catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. This protein can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. Recombinant human TPK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Thiamin pyrophosphokinase 1, HTPK1, PP20
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
29.8 kDa (267aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Nosaka K., et al. (2001) Biochim. Biophys. Acta. 1517:293-297; ; Zhao R., et al. (2004) Biochim. Biophys. Acta. 1517:320-322
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_071890
Uniprot Accession Number
Q9H3S4
Species
Human
AA Sequence
Frequently Asked Questions
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