Recombinant human TIFA protein

TIFA, also known as TRAF-interacting protein with FHA domain-containing protein A, is an adapter protein which mediates the IRAK1 and TRAF6 interaction following IL-1 stimulation, resulting in the downstream activation of NF-kappa-B and AP-1 pathways. This protein induces the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism. Recombinant human TIFA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

TRAF-interacting protein with FHA domain-containing protein A, T2BP, T6BP, TIFAA

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

24.0 kDa (208aa) confirmed by MALDI-TOF

Additionnal Information

TIFA, TRAF-interacting protein with FHA domain-containing protein A, T2BP, T6BP, TIFAA, ATGP1414-10 µg, ATGP1414-20 µg, ATGP1414-50 µg, ATGP1414-100 µg, ATGP1414-250 µg, ATGP1414-500 µg, ATGP1414-1 mg, ATGP1414-10, ATGP1414-20, ATGP1414-50, ATGP1414-100, ATGP1414-250, ATGP1414-500, ATGP1414-1

References & Citations

Takatsuna H., et al. (2003) J. Biol. Chem. 278:12144-12150; ; Ea C.-K., et al. (2004) Proc. Natl. Acad. Sci. u.S.A. 101:15318-15323

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_443096.1

Uniprot Accession Number

Q96CG3

Species

Human

AA Sequence

MTSFED ADTEETVTCL QMTVYHPGQL QCGIFQSISF NREKLPSSEV VKFGRNSNIC HYTFQDKQVS RVQFSLQLFK KFNSSVLSFE IKNMSKKTNL IVDSRELGYL NKMDLPYRCM VRFGEYQFLM EKEDGESLEF FETQFILSPR SLLQENNWPP HRPIPEYGTY SLCSSQSSSP TEMDENES