Recombinant human PIR-B protein

PIR, also known as pirin, is a member of the cupin superfamily. PIR is an Fe (II) -containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Recombinant human PIR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Pirin, Probable quercetin 2,3-dioxygenase PIR

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

34.3 kDa (310aa) confirmed by MALDI-TOF

Additionnal Information

PIR-B, Pirin, Probable quercetin 2,3-dioxygenase PIR, ATGP1413-10 µg, ATGP1413-20 µg, ATGP1413-50 µg, ATGP1413-100 µg, ATGP1413-250 µg, ATGP1413-500 µg, ATGP1413-1 mg, ATGP1413-10, ATGP1413-20, ATGP1413-50, ATGP1413-100, ATGP1413-250, ATGP1413-500, ATGP1413-1

References & Citations

Gelbman B.D., et al. (2007) Respir. Res. 8:10-10; ; Licciulli S., et al. (2010) Leukemia. 24:429-437

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001018119

Uniprot Accession Number

O00625

Species

Human

AA Sequence

MGSSKKVTLS VLSREQSEGV GARVRRSIGR PELKNLDPFL LFDEFKGGRP GGFPDHPHRG FETVSYLLEG GSMAHEDFCG HTGKMNPGDL QWMTAGRGIL HAEMPCSEEP AHGLQLWVNL RSSEKMVEPQ YQELKSEEIP KPSKDGVTVA VISGEALGIK SKVYTRTPTL YLDFKLDPGA KHSQPIPKGW TSFIYTISGD VYIGPDDAQQ KIEPHHTAVL GEGDSVQVEN KDPKRSHFVL IAGEPLREPV IQHGPFVMNT NEEISQAILD FRNAKNGFER AKTWKSKIGN