Recombinant human PIR-B protein
PIR, also known as pirin, is a member of the cupin superfamily. PIR is an Fe (II) -containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Recombinant human PIR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Pirin, Probable quercetin 2,3-dioxygenase PIR
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
34.3 kDa (310aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Gelbman B.D., et al. (2007) Respir. Res. 8:10-10; ; Licciulli S., et al. (2010) Leukemia. 24:429-437
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_001018119
Uniprot Accession Number
O00625
Species
Human
AA Sequence
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