Recombinant human NKIRAS1 protein
NKIRAS1, also known as NF-kappa-B inhibitor-interacting Ras-like protein 1, belongs to the small GTPase superfamily. NKIRAS1 is atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. It may act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB. Recombinant human NKIRAS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
NF-kappa-B inhibitor-interacting Ras-like protein 1, kappaB-Ras1, KBRAS1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
23.8 kDa (212aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Fenwick C., et al. (2000) Science. 287:869-873; ; Chen Y., et al. (2003) J. Biol. Chem. 278:23101-23106
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_065078.1
Uniprot Accession Number
Q9NYS0
Species
Human
AA Sequence
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