Recombinant human ANAPC13 protein
ANAPC13, also known as anaphase-promoting complex subunit 13, is component of the anaphase promoting complex, a large ubiquitin-protein ligase that controls cell cycle progression by regulating the degradation of cell cycle regulators such as B-type cyclins. This protein is evolutionarily conserved and is required for the integrity and ubiquitin ligase activity of the anaphase promoting complex Recombinant human ANAPC13 protein fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Anaphase-promoting complex subunit 13, APC13, SWM1
Expression System
E.coli
Antigen Species
Human
Tag
T7-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
10 kDa (89aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Schwickart M., et al. (2004) Mol. Cell. Biol. 24:3562-3576; ; Jin L., et al. (2008) Cell. 133:653-665
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl
Scientific Category
Ubiquitination
NCBI Accession Number
NP_056206
Uniprot Accession Number
Q9BS18
Species
Human
AA Sequence
MASMTGGQQM GRGSHMDSEV QRDGRILDLI DDAWREDKLP YEDVAIPLNE LPEPEQDNGG TTESVKEQEM KWTDLALQYL HENVPPIGN
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