Recombinant human TRAM/TICAM2 protein

Toll TIR domain-containing adapter molecule 2, also known as TICAM2, is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs) . TICAM-2, a cytoplasmic protein, physically bridges TLR4 and TICAM-1 and functionally transmits LPS-TLR4 signaling to TICAM-1, which in turn activates IRF-3. It is involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Recombinant human TICAM2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

TIR domain-containing adapter molecule 2, TICAM-2, TIRAP3, TIRP, TRAM

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

29.4 kDa (259aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

TICAM2, TIR domain-containing adapter molecule 2, TICAM-2, TIRAP3, TIRP, TRAM, ATGP1402-10 µg, ATGP1402-20 µg, ATGP1402-50 µg, ATGP1402-100 µg, ATGP1402-250 µg, ATGP1402-500 µg, ATGP1402-1 mg, ATGP1402-10, ATGP1402-20, ATGP1402-50, ATGP1402-100, ATGP1402-250, ATGP1402-500, ATGP1402-1

References & Citations

Yamamoto M., et al. (2002) J Immunol. 169:6668-6672.; ; Oshiumi H., et al. (2003) J Biol Chem. 278:49751-49762.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Immunology

NCBI Accession Number

NP_067681.1

Uniprot Accession Number

Q86XR7

Species

Human

AA Sequence

MGIGKS KINSCPLSLS WGKRHSVDTS PGYHESDSKK SEDLSLCNVA EHSNTTEGPT GKQEGAQSVE EMFEEEAEEE VFLKFVILHA EDDTDEALRV QNLLQDDFGI KPGIIFAEMP CGRQHLQNLD DAVNGSAWTI LLLTENFLRD TWCNFQFYTS LMNSVNRQHK YNSVIPMRPL NNPLPRERTP FALQTINALE EESRGFPTQV ERIFQESVYK TQQTIWKETR NMVQRQFIA