Recombinant human SSU72 protein

SSu72 RNA polymerase II CTD phosphatase homolog, also known as SSu72, is a highly conserved homologue of yeast Ssu72, a CTD phosphatase and a component of the polyadenylation/ termination machinery. Existing as multiple alternatively spliced isoforms, SSu72 interacts with TFIIB, Rb and DNAM-1 and functions to catalyze the dephosphorylation of target proteins, possibly playing a role in RNA processing and termination via dephosphorylation of Pol II. Recombinant human SSu72 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

SSu72 RNA polymerase II CTD phosphatase homolog, HSPC182, PNAS-120, RNA polymerase II subunit A C-terminal domain phosphatase SSU72, CTD phosphatase SSU72

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

25 kDa (217aa) confirmed by MALDI-TOF

Additionnal Information

SSu72, SSu72 RNA polymerase II CTD phosphatase homolog, HSPC182, PNAS-120, ATGP1401-10 µg, ATGP1401-20 µg, ATGP1401-50 µg, ATGP1401-100 µg, ATGP1401-250 µg, ATGP1401-500 µg, ATGP1401-1 mg, ATGP1401-10, ATGP1401-20, ATGP1401-50, ATGP1401-100, ATGP1401-250, ATGP1401-500, ATGP1401-1

References & Citations

Dichtl B., et al. (2002) Mol Cell. 10:1139-1150.; ; Krishnamurthy S., et al. (2004) Mol Cell. 14:387-394.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_054907

Uniprot Accession Number

Q9NP77

Species

Human

AA Sequence

MPSSPLR VAVVCSSNQN RSMEAHNILS KRGFSVRSFG TGTHVKLPGP APDKPNVYDF KTTYDQMYND LLRKDKELYT QNGILHMLDR NKRIKPRPER FQNCKDLFDL ILTCEERVYD QVVEDLNSRE QETCQPVHVV NVDIQDNHEE ATLGAFLICE LCQCIQHTED MENEIDELLQ EFEEKSGRTF LHTVCFY