Recombinant human Inorganic Pyrophosphatase/PPA1 protein
PPA1 (Pyrophosphatase) belongs to the PPase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. The hydrolysis of inorganic pyrophosphate (PPi) to two phosphate ions is utilized in many biochemical pathways to render reactions effectively irreversible. Inorganic pyrophosphatase catalyzes this hydrolysis reaction in the early steps of lipid degradation, a prominent example of this phenomenon. By promoting the rapid hydrolysis of pyrophosphate (PPi), Inorganic pyrophosphatase provides the driving force for the activation of fatty acids destined for oxidation. Recombinant human PPA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
35.2 kDa (313aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Carman GM, et al. (2006) Trends Biochem. Sci. 31 (12) : 694-9.; ; usui Y, et al. (2010) J. Dent. Res. 89 (5) : 504-9.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_066952
Uniprot Accession Number
Q15181
Species
Human
AA Sequence
Frequently Asked Questions
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