Recombinant human Inorganic Pyrophosphatase/PPA1 protein

PPA1 (Pyrophosphatase) belongs to the PPase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. The hydrolysis of inorganic pyrophosphate (PPi) to two phosphate ions is utilized in many biochemical pathways to render reactions effectively irreversible. Inorganic pyrophosphatase catalyzes this hydrolysis reaction in the early steps of lipid degradation, a prominent example of this phenomenon. By promoting the rapid hydrolysis of pyrophosphate (PPi), Inorganic pyrophosphatase provides the driving force for the activation of fatty acids destined for oxidation. Recombinant human PPA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

35.2 kDa (313aa) confirmed by MALDI-TOF

Additionnal Information

Inorganic Pyrophosphatase, PPA1, Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061, ATGP1397-10 µg, ATGP1397-20 µg, ATGP1397-50 µg, ATGP1397-100 µg, ATGP1397-250 µg, ATGP1397-500 µg, ATGP1397-1 mg, ATGP1397-10, ATGP1397-20, ATGP1397-50, ATGP1397-100, ATGP1397-250, ATGP1397-500, ATGP1397-1

References & Citations

Carman GM, et al. (2006) Trends Biochem. Sci. 31 (12) : 694-9.; ; usui Y, et al. (2010) J. Dent. Res. 89 (5) : 504-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_066952

Uniprot Accession Number

Q15181

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMSGFST EERAAPFSLE YRVFLKNEKG QYISPFHDIP IYADKDVFHM VVEVPRWSNA KMEIATKDPL NPIKQDVKKG KLRYVANLFP YKGYIWNYGA IPQTWEDPGH NDKHTGCCGD NDPIDVCEIG SKVCARGEII GVKVLGILAM IDEGETDWKV IAINVDDPDA ANYNDINDVK RLKPGYLEAT VDWFRRYKVP DGKPENEFAF NAEFKDKDFA IDIIKSTHDH WKALVTKKTN GKGISCMNTT LSESPFKCDP DAARAIVDAL PPPCESACTV PTDVDKWFHH QKN