Recombinant human ARH/LDLRAP1 protein
LDLRAP1 (Low density lipoprotein receptor adapter protein 1) is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. LDLRAP1 is a cytosolic adaptor that couples LDLR to endocytic machinery. Defects in LDLRAP1 are the cause of autosomal recessive hypercholesterolemia (ARH) . ARH is a disorder caused by defective internalization of LDL receptors (LDLR) in the liver. Recombinant human LDLRAP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Low density lipoprotein receptor adaptor protein 1, ARH, ARH1, ARH2, FHCB1, FHCB2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
36.1 kDa (328aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Garcia C.K., et al. (2001) Science 292:1394-1398; ; Mishra S.K., et al. (2005) J. Biol. Chem. 280:19270-19280
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
AAH29770
Uniprot Accession Number
Q5SW96
Species
Human
AA Sequence
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
