Recombinant human ARH/LDLRAP1 protein

LDLRAP1 (Low density lipoprotein receptor adapter protein 1) is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. LDLRAP1 is a cytosolic adaptor that couples LDLR to endocytic machinery. Defects in LDLRAP1 are the cause of autosomal recessive hypercholesterolemia (ARH) . ARH is a disorder caused by defective internalization of LDL receptors (LDLR) in the liver. Recombinant human LDLRAP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Low density lipoprotein receptor adaptor protein 1, ARH, ARH1, ARH2, FHCB1, FHCB2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.1 kDa (328aa) confirmed by MALDI-TOF

Additionnal Information

LDLRAP1, Low density lipoprotein receptor adaptor protein 1, ARH, ARH1, ARH2, FHCB1, FHCB2, ATGP1396-10 µg, ATGP1396-20 µg, ATGP1396-50 µg, ATGP1396-100 µg, ATGP1396-250 µg, ATGP1396-500 µg, ATGP1396-1 mg, ATGP1396-10, ATGP1396-20, ATGP1396-50, ATGP1396-100, ATGP1396-250, ATGP1396-500, ATGP1396-1

References & Citations

Garcia C.K., et al. (2001) Science 292:1394-1398; ; Mishra S.K., et al. (2005) J. Biol. Chem. 280:19270-19280

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

AAH29770

Uniprot Accession Number

Q5SW96

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MDALKSAGRA LIRSPSLAKQ SWGGGGRHRK LPENWTDTRE TLLEGMLFSL KYLGMTLVEQ PKGEELSAAA IKRIVATAKA SGKKLQKVTL KVSPRGIILT DNLTNQLIEN VSIYRISYCT ADKMHDKVFA YIAQSQHNQS LECHAFLCTK RKMAQAVTLT VAQAFKVAFE FWQVSKEEKE KRDKASQEGG DVLGARQDCT PPLKSLVATG NLLDLEETAK APLSTVSANT TNMDEVPRPQ ALSGSSVVWE LDDGLDEAFS RLAQSRTNPQ VLDTGLTAQD MHYAQCLSPV DWDKPDSSGT EQDDLFSF