Recombinant e.coli coaA protein
CoaA (pantothenate kinase) belongs to the prokaryotic pantothenate kinase family. Pantothenate kinase (PanK; CoaA) is the first enzyme in the Coenzyme A biosynthetic pathway. It phophorylates pantothenate (vitamin B5) to form 4'-phosphopantothenate. Regulation of pantothenate kinase activity by feedback inhibition is the critical factor controlling the intracellular CoA concentration. Recombinant E. coli coaA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Pantothenate kinase, panK, rts, ts-9
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
38.9 kDa (340aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Song W.-J., et al. (1992) J.Bacteriol. 174:6411-6417; ; Song W.-J., et al. (1992) J.Bacteriol. 174:1705-1706
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_418405
Uniprot Accession Number
P0A6I3
Species
E.coli
AA Sequence
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