Recombinant e.coli coaA protein

CoaA (pantothenate kinase) belongs to the prokaryotic pantothenate kinase family. Pantothenate kinase (PanK; CoaA) is the first enzyme in the Coenzyme A biosynthetic pathway. It phophorylates pantothenate (vitamin B5) to form 4'-phosphopantothenate. Regulation of pantothenate kinase activity by feedback inhibition is the critical factor controlling the intracellular CoA concentration. Recombinant E. coli coaA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Pantothenate kinase, panK, rts, ts-9

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

38.9 kDa (340aa) confirmed by MALDI-TOF

Additionnal Information

CoaA, pantothenate kinase, panK, rts, ts-9, ATGP1395-10 µg, ATGP1395-20 µg, ATGP1395-50 µg, ATGP1395-100 µg, ATGP1395-250 µg, ATGP1395-500 µg, ATGP1395-1 mg, ATGP1395-10, ATGP1395-20, ATGP1395-50, ATGP1395-100, ATGP1395-250, ATGP1395-500, ATGP1395-1

References & Citations

Song W.-J., et al. (1992) J.Bacteriol. 174:6411-6417; ; Song W.-J., et al. (1992) J.Bacteriol. 174:1705-1706

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_418405

Uniprot Accession Number

P0A6I3

Species

E.coli

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMSIKEQ TLMTPYLQFD RNQWAALRDS VPMTLSEDEI ARLKGINEDL SLEEVAEIYL PLSRLLNFYI SSNLRRQAVL EQFLGTNGQR IPYIISIAGS VAVGKSTTAR VLQALLSRWP EHRRVELITT DGFLHPNQVL KERGLMKKKG FPESYDMHRL VKFVSDLKSG VPNVTAPVYS HLIYDVIPDG DKTVVQPDIL ILEGLNVLQS GMDYPHDPHH VFVSDFVDFS IYVDAPEDLL QTWYINRFLK FREGAFTDPD SYFHNYAKLT KEEAIKTAMT LWKEINWLNL KQNILPTRER ASLILTKSAN HAVEEVRLRK