Recombinant human ABHD14B protein
ABHD14B (abhydrolase domain containing 14B) belongs to the AB hydrolase superfamily. This protein contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing (ABHD) gene subfamily is comprised of 15 mostly uncharacterized members. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) . It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Abhydrolase domain containing 14B, CIB
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
25kDa (234aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Padmanabhan B., et al. (2004) J. Biol. Chem. 279:9615-9624; ; Holmquist, M. (2000) Curr. Protein Pept. Sci. 1: 209-235.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_116139
Uniprot Accession Number
Q96IU4
Species
Human
AA Sequence
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