Recombinant human DUSP21 protein
DuSP21 (Dual specificity protein phosphatase 21) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases (DuSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. This enzyme can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. It is exclusively expressed in testis where is preferentially dephosphorylates phosphotyrosine residues in MAPK peptides. Recombinant human DuSP21 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Dual specificity protein phosphatase 21, Low molecular weight dual specificity phosphatase 21, LMW-DSP21
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
24.1 kDa (214aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hood, K.L., et al. (2002) Biochem. Biophys. Res. Commun. 298: 545-551.; ; Keyse, S.M. (1995) Biochim. Biophys. Acta 1265: 152-160.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200 mM NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_071359
Uniprot Accession Number
Q9H596
Species
Human
AA Sequence
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