Recombinant human DUSP10 protein

DuSP10 (Dual specificity protein phosphatase 10) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases (DuSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. DuSP10 has been shown to interact with MAPK14 and MAPK8. When expressed in mammalian cells, DuSP10 blocks the enzymatic activation of MAP kinases with the selectivity p38 approximately JNK/SAPK >> ERK. Recombinant human DuSP10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 600 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Product Name Alternative

Dual specificity protein phosphatase 10, MKP-5, MKP5, Mitogen-activated protein kinase phosphatase 5, MAP kinase phosphatase 5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 600unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Molecular Weight

40.4 kDa (359aa) confirmed by MALDI-TOF

Additionnal Information

DUSP10, Dual specificity protein phosphatase 10, MKP-5, MKP5, ATGP1392-10 µg, ATGP1392-20 µg, ATGP1392-50 µg, ATGP1392-100 µg, ATGP1392-250 µg, ATGP1392-500 µg, ATGP1392-1 mg, ATGP1392-10, ATGP1392-20, ATGP1392-50, ATGP1392-100, ATGP1392-250, ATGP1392-500, ATGP1392-1

References & Citations

Theodosiou A, et al. (1999) Oncogene. 18 (50) :6981-8.; ; Jeong DG, et al. (2006) J Mol Biol. 360 (5) :946-55.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 200 mM NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_009138

Uniprot Accession Number

Q9Y6W6

Species

Human

AA Sequence

IIYPN DLAKKMTKCS KSHLPSQGPV IIDCRPFMEY NKSHIQGAVH INCADKISRR RLQQGKITVL DLISCREGKD SFKRIFSKEI IVYDENTNEP SRVMPSQPLH IVLESLKREG KEPLVLKGGL SSFKQNHENL CDNSLQLQEC REVGGGASAA SSLLPQPIPT TPDIENAELT PILPFLFLGN EQDAQDLDTM QRLNIGYVIN VTTHLPLYHY EKGLFNYKRL PATDSNKQNL RQYFEEAFEF IEEAHQCGKG LLIHCQAGVS RSATIVIAYL MKHTRMTMTD AYKFVKGKRP IISPNLNFMG QLLEFEEDLN NGVTPRILTP KLMGVETVV