Recombinant human Importin alpha 2/KPNA2 protein

Karyopherin alpha2 (KPNA2) is a member of the karyopherin alpha family. This protein plays a key role in the nuclear import of proteins with a classical nuclear localization signal (NLS) . KPNA2 bound in vitro specifically and directly to substrates containing either a simple or bipartite NLS motif. It promoted docking of import substrates to the nuclear envelope and together with recombinant human Ran reconstituted complete nuclear protein import. Recombinant human KPNA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Importin subunit alpha-2, IPOA1, QIP2, SRP1alpha

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

60.4 kDa (553aa)

Additionnal Information

Importin a2, KPNA2, Importin subunit alpha-2, IPOA1, QIP2, SRP1alpha, ATGP1389-10 µg, ATGP1389-20 µg, ATGP1389-50 µg, ATGP1389-100 µg, ATGP1389-250 µg, ATGP1389-500 µg, ATGP1389-1 mg, ATGP1389-10, ATGP1389-20, ATGP1389-50, ATGP1389-100, ATGP1389-250, ATGP1389-500, ATGP1389-1

References & Citations

Moroianu J., et al. (1995) Proc. Natl. Acad. Sci. u.S.A. 92:6532-6536; ; Braem C.V., et al. (2002) J. Biol. Chem. 277:19673-19678

Other References

Song KH, et al. Induction of immunogenic cell death by radiation-upregulated karyopherin alpha 2 in vitro. (Eur J Cell Biol. 2016) {https://pubmed.ncbi.nlm.nih.gov/27107455/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

AAH05978.1

Uniprot Accession Number

P52292

Species

Human

AA Sequence

MSTNEN ANTPAARLHR FKNKGKDSTE MRRRRIEVNV ELRKAKKDDQ MLKRRNVSSF PDDATSPLQE NRNNQGTVNW SVDDIVKGIN SSNVENQLQA TQAARKLLSR EKQPPIDNII RAGLIPKFVS FLGRTDCSPI QFESAWALTN IASGTSEQTK VVVDGGAIPA FISLLASPHA HISEQAVWAL GNIAGDGSVF RDLVIKYGAV DPLLALLAVP DMSSLACGYL RNLTWTLSNL CRNKNPAPPI DAVEQILPTL VRLLHHDDPE VLADTCWAIS YLTDGPNERI GMVVKTGVVP QLVKLLGASE LPIVTPALRA IGNIVTGTDE QTQVVIDAGA LAVFPSLLTN PKTNIQKEAT WTMSNITAGR QDQIQQVVNH GLVPFLVSVL SKADFKTQKE AVWAVTNYTS GGTVEQIVYL VHCGIIEPLM NLLTAKDTKI ILVILDAISN IFQAAENLGE TEKLSIMIEE CGGLDKIEAL QNHENESVYK ASLSLIEKYF SVEEEEDQNV VPETTSEGYT FQVQDGAPGT FNF