Recombinant human Importin alpha 2/KPNA2 protein
Karyopherin alpha2 (KPNA2) is a member of the karyopherin alpha family. This protein plays a key role in the nuclear import of proteins with a classical nuclear localization signal (NLS) . KPNA2 bound in vitro specifically and directly to substrates containing either a simple or bipartite NLS motif. It promoted docking of import substrates to the nuclear envelope and together with recombinant human Ran reconstituted complete nuclear protein import. Recombinant human KPNA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Importin subunit alpha-2, IPOA1, QIP2, SRP1alpha
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
60.4 kDa (553aa)
Additionnal Information
References & Citations
Moroianu J., et al. (1995) Proc. Natl. Acad. Sci. u.S.A. 92:6532-6536; ; Braem C.V., et al. (2002) J. Biol. Chem. 277:19673-19678
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
AAH05978.1
Uniprot Accession Number
P52292
Species
Human
AA Sequence
Frequently Asked Questions
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