Recombinant human LASP1 protein

LASP1, also known as LIM and SH3 domain protein 1, plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types. Recombinant human LASP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

LIM and SH3 domain protein 1, Lasp-1, MLN50

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

32.3 kDa (285aa) confirmed by MALDI-TOF

Additionnal Information

LASP1, LIM and SH3 domain protein 1, Lasp-1, MLN50, ATGP1386-10 µg, ATGP1386-20 µg, ATGP1386-50 µg, ATGP1386-100 µg, ATGP1386-250 µg, ATGP1386-500 µg, ATGP1386-1 mg, ATGP1386-10, ATGP1386-20, ATGP1386-50, ATGP1386-100, ATGP1386-250, ATGP1386-500, ATGP1386-1

References & Citations

Tomasetto C., et al. (1995) FEBS Lett. 373:245-249; ; Choudhary C., et al. (2002) Science. 325:834-840

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_006139

Uniprot Accession Number

Q14847

Species

Human

AA Sequence

MNPNCA RCGKIVYPTE KVNCLDKFWH KACFHCETCK MTLNMKNYKG YEKKPYCNAH YPKQSFTMVA DTPENLRLKQ QSELQSQVRY KEEFEKNKGK GFSVVADTPE LQRIKKTQDQ ISNIKYHEEF EKSRMGPSGG EGMEPERRDS QDGSSYRRPL EQQQPHHIPT SAPVYQQPQQ QPVAQSYGGY KEPAAPVSIQ RSAPGGGGKR YRAVYDYSAA DEDEVSFQDG DTIVNVQQID DGWMYGTVER TGDTGMLPAN YVEAI