Recombinant human ZFAND3 protein

ZFAND3, also known as AN1-type zinc finger protein 3, contains DNA-binding domain and has a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZFAND3 is a 251 amino acid protein containing two AN1-type zinc fingers and two uIM (ubiquitin-interacting motif) repeats. Conserved in animals and plants, the AN1-type zinc finger domain is often found in proteins that contain a ubiquitin-like domain, which suggests a role in the ubiquitination pathway. Recombinant human ZFAND3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

AN1-type zinc finger protein 3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.7 kDa (251aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

ZFAND3, AN1-type zinc finger protein 3, ATGP1385-10 µg, ATGP1385-20 µg, ATGP1385-50 µg, ATGP1385-100 µg, ATGP1385-250 µg, ATGP1385-500 µg, ATGP1385-1 mg, ATGP1385-10, ATGP1385-20, ATGP1385-50, ATGP1385-100, ATGP1385-250, ATGP1385-500, ATGP1385-1

References & Citations

Klug A. et al. (1999) J. Mol. Biol. 293: 215-218.; ; Laity J.H. et al. (2007) Curr. Opin. Struct. Biol. 11: 39-46.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_068762

Uniprot Accession Number

Q9H8U3

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMGDAGS ERSKAPSLPP RCPCGFWGSS KTMNLCSKCF ADFQKKQPDD DSAPSTSNSQ SDLFSEETTS DNNNTSITTP TLSPSQQPLP TELNVTSPSK EECGPCTDTA HVSLITPTKR SCGTDSQSEN EASPVKRPRL LENTERSEET SRSKQKSRRR CFQCQTKLEL VQQELGSCRC GYVFCMLHRL PEQHDCTFDH MGRGREEAIM KMVKLDRKVG RSCQRIGEGC S