Recombinant human DCTPP1 protein

DCTPP1, also known as dCTP pyrophosphatase 1, contains an epilepsy-associated repeat (EAR) domain that is predicted to participate in protein-protein interactions, most likely involved in ligand recognition. This protein functions to hydrolyze abnormal nucleotide triphosphates (NTPs) in cancer cells that, if unregulated, could be incorporated into nascent DNA or RNA. Recombinant human DCTPP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Deoxycytidine-triphosphatase 1, dCTPase 1, dCTP pyrophosphatase 1, CDA03, RS21C6, XTP3TPA

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

21.2 kDa (194aa) confirmed by MALDI-TOF

Additionnal Information

DCTPP1, dCTP pyrophosphatase 1, CDA03, RS21C6, XTP3TPA, ATGP1381-10 µg, ATGP1381-20 µg, ATGP1381-50 µg, ATGP1381-100 µg, ATGP1381-250 µg, ATGP1381-500 µg, ATGP1381-1 mg, ATGP1381-10, ATGP1381-20, ATGP1381-50, ATGP1381-100, ATGP1381-250, ATGP1381-500, ATGP1381-1

References & Citations

Moroz O.V. et al. (2005) J. Mol. Biol. 347: 243-255.; ; Rual J.F. et al. (2005) Nature 437:1173-1178.

Other References

Kawamura T, et al. Proteomic profiling of small-molecule inhibitors reveals dispensability of MTH1 for cancer cell survival. (Sci Rep. 2016) {https://pubmed.ncbi.nlm.nih.gov/27210421/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_077001

Uniprot Accession Number

Q9H773

Species

Human

AA Sequence

MSVAGG EIRGDTGGED TAAPGRFSFS PEPTLEDIRR LHAEFAAERD WEQFHQPRNL LLALVGEVGE LAELFQWKTD GEPGPQGWSP RERAALQEEL SDVLIYLVAL AARCRVDLPL AVLSKMDINR RRYPAHLARS SSRKYTELPH GAISEDQAVG PADIPCDSTG QTST