Recombinant human CHMP4A protein

CHMP4A (Chromatin-modifying protein 4a) belongs to the SNF7 family and functions as chromatin-modifying proteins. It probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. Also during HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins via the association of CHMP4 and a protein recruited by HIV-1 p6, which is present in viral Gag assembly and budding. CHMP4A expressed at higher level in heart, kidney, liver and skeletal muscle. Recombinant human CHMP4A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Charged multivesicular body protein 4A, Chromatin-modifying protein 4a, CHMP4a, SNF7 homolog associated with Alix-2, SNF7-1, hSnf-1, Vacuolar protein sorting-associated protein 32-1, Vps32-1, hVps32-1, HSPC134, VPS32A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

31.9kDa (285aa) confirmed by MALDI-TOF

Additionnal Information

CHMP4A, chromatin modifying protein 4A, CHMP4, CHMP4B, HSPC134, SHAX2, SNF7, SNF7-1, VPS32-1, VPS32A, ATGP1376-10 µg, ATGP1376-20 µg, ATGP1376-50 µg, ATGP1376-100 µg, ATGP1376-250 µg, ATGP1376-500 µg, ATGP1376-1 mg, ATGP1376-10, ATGP1376-20, ATGP1376-50, ATGP1376-100, ATGP1376-250, ATGP1376-500, ATGP1376-1

References & Citations

Katoh K., et al. (2004) Arch. Biochem. Biophys. 421:159-165; ; Strack B., et al (2003) Cell 114:689-699

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 200 mM NaCl, 0.1mM PMSF, 1mM EDTA

Scientific Category

Metabolism

NCBI Accession Number

NP_054888

Uniprot Accession Number

Q9BY43

Species

Human

AA Sequence

MSRRRPEDGL GKAGPCVMRH HPPRSKAEVW RTLRGGGGRG ELAMSGLGRL FGKGKKEKGP TPEEAIQKLK ETEKILIKKQ EFLEQKIQQE LQTAKKYGTK NKRAALQALR RKKRFEQQLA QTDGTLSTLE FQREAIENAT TNAEVLRTME LAAQSMKKAY QDMDIDKVDE LMTDITEQQE VAQQISDAIS RPMGFGDDVD EDELLEELEE LEQEELAQEL LNVGDKEEEP SVKLPSVPST HLPAGPAPKV DEDEEALKQL AEWVS