Recombinant human ATF1 protein
Activating transcription factor 1, also known as ATF1, is a cyclic-AMP dependent transcription factor. ATF1 is expressed in a wide variety of cell types and is capable of dimerizing with CREB. MSK1 and MSK2 protein kinases are required for the stress-induced phosphorylation of transcription factors CREB and ATF1 in primary embryonic fibroblasts. Epidermal growth factor induction of c-jun expression requires ATF1 and MEF2 sites in the c-jun promoter. Recombinant human ATF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Activating transcription factor 1, EWS-ATF1, FuS/ATF-1, TREB36
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
31.8 kDa (295aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Lin Y S., et al. (1988) Proc Natl Acad Sci uSA. 85:3396-3400.; ; Gupta P., et al. (2002) J Biol Chem. 277:50550-50556.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 0.2M NaCl, 2mM EDTA
Scientific Category
Signal Transduction
NCBI Accession Number
NP_005162.1
Uniprot Accession Number
P18846
Species
Human
AA Sequence
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