Recombinant human PCK1 protein
Phosphoenolpyruvate carboxykinase 1, also known as PCK1, is a main control point for the regulation of gluconeogenesis. PCK1 plays an important role in this process by stimulating hepatic glucose production. The expression of PCK1 can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Modulation of the signals governing PCK1 levels presents a potential therapeutic approach to the treatment of Insulin resistance and consequently obesity. Recombinant human PCK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Phosphoenolpyruvate carboxykinase 1, Phosphoenolpyruvate carboxykinase 1, PEPCK-C, PEPCK1, PEPCKC
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
71.7 kDa (646aa)
Additionnal Information
References & Citations
Barthel A., et al. (2003) Am J Physiol Endocrinol Metab. 285:685-692.; ; Wang Y., et al. (1991) J Cell Physiol. 147:374-382.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Metabolism
NCBI Accession Number
AAH23978.1
Uniprot Accession Number
P35558
Species
Human
AA Sequence
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